Cell Line Development

Everyone
  • 12 videos | 59m 15s
  • Includes Assessment
Learn about cell line development

WHAT YOU WILL LEARN

  • Understand the basics of cell line development
    Understand the history of recombinant dna
    Understand the design of dna vectors for protein expression
    Understand how to select a host for production
    Understand the details of selecting a host
    Understand vector design for mammalian hosts
  • Understand more about vector design
    Understand clonal selection
    Understand how cell line development works in real life
    Understand automation in clonal selection
    Understand cell banking
    Understand sharp's role in the topics covered

IN THIS COURSE

  • 4m 35s
    In the following video, Professor Love will talk about the steps in cell line development and highlight why choosing the correct host cell line has an impact on nearly all areas of manufacturing. FREE ACCESS
  • 5m 15s
    In the following video, Professor Love tells you about the history behind the invention of recombinant DNA technology. FREE ACCESS
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    3.  Design Of DNA Vectors For Protein Expression
    4m 4s
    In the following video, Professor Love will discuss in more detail the common features of expression vectors used for heterologous protein expression. FREE ACCESS
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    4.  Selection Of A Host For Production: Part 1
    2m 58s
    In the following video, Professor Love discusses some of the key factors to consider when choosing a type of cell to manufacture a biologic drug. FREE ACCESS
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    5.  Selection Of A Host For Production: Part 2
    5m 23s
    In the following video, Professor Love will discuss these topics in more detail as well as describe the origin of one of the most common cell lines used in modern biopharmaceutical manufacturing, Chinese Hamster Ovary, or CHO, cells. FREE ACCESS
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    6.  Vector Design For Mammalian Hosts: Part 1
    6m 31s
    In the following video, Professor Love describes the elements of vector design in mammalian hosts in more detail. You will also hear from MIT Professor Phil Sharp about one of the first selectable markers used in mammalian cells. FREE ACCESS
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    7.  Vector Design For Mammalian Hosts: Part 2
    4m 37s
    In the following video, Professor Love will discuss these topics in more detail and show you examples of each strategy to improve protein production. FREE ACCESS
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    8.  Clonal Selection
    5m 50s
    In the following video, Professor Love discusses clonal selection topics in more detail. At the end, you will be able to identify important criteria for selecting an ideal clone. FREE ACCESS
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    9.  Cell Line Development In Action
    5m 58s
    Now that we know how to select our clones, let's find out what happens in a Cell Line Development lab. In the following video, Professor Love is on location at Pfizer in Andover, MA to talk to Principal Scientist Linda Francullo about the process that they use to develop their cells. FREE ACCESS
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    10.  Automation For Clonal Selection
    3m 18s
    In the following video, Professor Love visits the Pfizer Pilot Plant in Andover, MA. There, he talks to former Principal Scientist Tom Nelson (now Data Scientist, Computational Biology and Machine Learning at Biocomputronics) about automation for clonal selection. FREE ACCESS
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    11.  Cell Banking
    4m 1s
    In this video, Professor Love discusses both how to prepare, and how to thoroughly characterize, a master cell bank in more detail. FREE ACCESS
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    12.  Meet The Experts: Phillip A. Sharp
    6m 45s
    Meet a leading expert in the field: Phillip A. Sharp FREE ACCESS

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