Chromatography & Putting It All Together
Everyone
- 9 videos | 45m 8s
- Includes Assessment
Learn about chromatography and putting together many topics of downstream processing
WHAT YOU WILL LEARN
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Understand the basics of chromatographyUnderstand chromatographic operationUnderstand gel filtration chromatographyUnderstand the remaining methods for chromatographyUnderstand capture chromatography in action
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Understand polishing chromatography in actionUnderstand how to purify insulinUnderstand how to purify monoclonal antibodiesUnderstand viral clearance in downstream purification
IN THIS COURSE
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5m 32sIn the following video, Dr. Barone discusses chromatography in more detail as well as explains how chromatography was invented. FREE ACCESS
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5m 44sIn the following video, Dr. Barone discusses some theoretical aspects of chromatographic operation as well as some of the history behind their development. FREE ACCESS
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5m 50sIn the following video, Dr. Barone discusses some key discoveries that enabled chromatography to be used to separate proteins. He also explains gel filtration in more detail. FREE ACCESS
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6m 13sIn this last segment about chromatography, Dr. Barone discusses each of the remaining methods in more detail and introduces some theoretical considerations when designing a chromatographic process. FREE ACCESS
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3m 17sWhat does a chromatography column actually look like? Let's go find out. In the following video, Dr. Barone is back at Pfizer with Scientist Mark Kivimaki discussing affinity chromatography for protein purification. FREE ACCESS
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1m 23sIn the steps of downstream processing, initial purification is followed by polishing. Polishing is often done using chromatography. In the following video, Dr. Barone and Pfizer's Mark Kivimaki go over the process of polishing chromatography for removal of impurities. FREE ACCESS
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3m 53sNow that we have all of the tools of downstream processing, let's finish our example of purifying insulin. In the final segment on the purification of insulin, Dr. Barone analyzes the remaining steps needed to obtain a pure insulin product. FREE ACCESS
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7m 11sIn the following video, Dr. Barone walks through the downstream processing steps for a monoclonal antibody produced from mammalian culture. FREE ACCESS
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6m 6sDr. Barone discusses the role downstream processing plays in the inactivation and removal of potential viruses. FREE ACCESS
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