Chromatography & Putting It All Together

  • 9 videos | 45m 8s
  • Includes Assessment
Learn about chromatography and putting together many topics of downstream processing


  • Understand the basics of chromatography
    Understand chromatographic operation
    Understand gel filtration chromatography
    Understand the remaining methods for chromatography
    Understand capture chromatography in action
  • Understand polishing chromatography in action
    Understand how to purify insulin
    Understand how to purify monoclonal antibodies
    Understand viral clearance in downstream purification


  • 5m 32s
    In the following video, Dr. Barone discusses chromatography in more detail as well as explains how chromatography was invented. FREE ACCESS
  • 5m 44s
    In the following video, Dr. Barone discusses some theoretical aspects of chromatographic operation as well as some of the history behind their development. FREE ACCESS
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    3.  Chromatography Part 3: Gel Filtration Chromatography
    5m 50s
    In the following video, Dr. Barone discusses some key discoveries that enabled chromatography to be used to separate proteins. He also explains gel filtration in more detail. FREE ACCESS
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    4.  Chromatography Part 4
    6m 13s
    In this last segment about chromatography, Dr. Barone discusses each of the remaining methods in more detail and introduces some theoretical considerations when designing a chromatographic process. FREE ACCESS
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    5.  Capture Chromatography In Action
    3m 17s
    What does a chromatography column actually look like? Let's go find out. In the following video, Dr. Barone is back at Pfizer with Scientist Mark Kivimaki discussing affinity chromatography for protein purification. FREE ACCESS
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    6.  Polishing Chromatography In Action
    1m 23s
    In the steps of downstream processing, initial purification is followed by polishing. Polishing is often done using chromatography. In the following video, Dr. Barone and Pfizer's Mark Kivimaki go over the process of polishing chromatography for removal of impurities. FREE ACCESS
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    7.  Purification Of Insulin
    3m 53s
    Now that we have all of the tools of downstream processing, let's finish our example of purifying insulin. In the final segment on the purification of insulin, Dr. Barone analyzes the remaining steps needed to obtain a pure insulin product. FREE ACCESS
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    8.  Purification Of Monoclonal Antibody
    7m 11s
    In the following video, Dr. Barone walks through the downstream processing steps for a monoclonal antibody produced from mammalian culture. FREE ACCESS
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    9.  Viral Clearance In Downstream Purification
    6m 6s
    Dr. Barone discusses the role downstream processing plays in the inactivation and removal of potential viruses. FREE ACCESS


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